Fig. 7.

Validation of key genes in the ceRNA network. The effect of cisplatin on the mRNA level of CELF2 (A), CFL2 (B), NEURL1B (C), PDIA5 (D), SRSF1 (E), and UNK (F) and KGN cells was cultured in the presence or absence of cisplatin (50 μM) for 24 h. The mRNA levels of CELF2, CFL2, NEURL1B, PDIA5, SRSF1, and UNK were determined by qPCR and normalized to GAPDH levels. ceRNA network: competitive endogenous RNA network; NC: negative control; POI: premature ovarian failure; qPCR: quantitative polymerase chain reaction. The data is expressed as the means±SD (n = 3). *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001