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. 2024 Jan 15;15(1):51. doi: 10.1038/s41419-024-06448-5

Fig. 5. Mitochondrial stress induces YAP dephosphorylation via both LATS-dependent and LATS-independent mechanisms.

Fig. 5

A LATS1/2 are primarily responsible for YAP phosphorylation. LATS1/2 KO and wild-type cells were serum-starved for 12 h, then treated with CCCP, analyzed by immunoblotting. B Mitochondrial stress is capable of activating YAP without LATS1/2. Cells were subjected to serum starvation or 2-DG (25 mM) treatment for 1 h, then treated with CCCP, followed by YAP immunoblotting analysis. C PP2A inhibition blocks LATS1/2-independent YAP activation. LATS1/2 KO cells were pretreated with 2-DG (25 mM) and LATS1/2 inhibitor LB-100 (5 μM) for 1 h and then treated with CCCP for 1 h. D Cells were serum-starved for 16 h, pretreated with LB-100 (5 μM), and given CCCP before immunoblotting. PP2Acα/β KO and wild-type cells were serum-starved for 12 h and subjected to CCCP treatment (1 or 3 h) for YAP immunoblotting (E), YAP/TAZ immunostaining (F), and qRT-PCR for the target genes (G). Scale bar: 50 μm. For A–G, HEK293 cells and 50 μM CCCP were used.