Fig. 7. Hepatocyte YAP/TAZ promotes acetaminophen-induced liver injury and inflammation.
A–G Yap/Tazfl/fl mice were treated with AAV8-TBG-Cre or AAV8-TBG-Null control virus to induce hepatocyte-specific KO. After 14 days the mice were fasted for 12 h, orally given APAP or vehicle, then sacrificed 24 h later. A Experimental scheme. B Immunoblot for YAP/TAZ in mice with Cre virus (Yap/TazHepKO) or control virus (WT). C Serum ALT. D Liver sections stained with H&E and fluorescently labeled with active caspase-3 antibody. Scale bars, 100 μm. E Heatmap showing APAP-induced genes from an RNA sequencing using the livers from mice. F qRT-PCR analysis in mouse liver. G Immunohistochemistry using F4/80 antibody. Wild-type samples were shared with Fig. 1D. Scale bar: 100 μm. H A proposed model for YAP/TAZ activation during drug-induced liver injury. For B, C, F, data represent the mean ± SEM. **P < 0.01, by Student’s t-test.