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. 2024 Jan 16;8:11. doi: 10.1038/s41698-024-00503-2

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© The Author(s) 2024

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — a Colony formation assay measuring cell viability in DU145 and PC3 cell lines (left) and histogram quantification (right). b Representative in vivo subcutaneous tumorigenesis images of the tumors of control group (left side in upper panel and upper side in lower panel) and circCCDC7(15,16,17,18,19) overexpression group (right side in upper panel and lower side in lower panel) using DU145. c, d Tumor weight and volume were measured after the tumors were surgically dissected. e, f Left panel: representative images of migration and invasion assays using DU145 (e) and PC3 (f) cells with or without circCCDC7(15,16,17,18,19) overexpression. Right panel: Histogram analysis of migrated and invaded cell counts. g Representative image of the Western blotting analysis of Slug, E-cad, and Claudin after circCCDC7(15,16,17,18,19) overexpression in DU145 and PC3 cells. The grayscale ratio of target protein to GAPDH was listed on top of the lanes. *p < 0.05, **p < 0.01, ***p < 0.001.