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. 2024 Jan 16;8:11. doi: 10.1038/s41698-024-00503-2

Fig. 5. CircCCDC7(15,16,17,18,19) suppresses the progression of PCa by its encoded protein.

Fig. 5

a Schematic diagram of circCCDC7(15,16,17,18,19) frame shift mutant which one base after the start codon (ATG) was deleted. b Schematic diagram of linearized circCCDC7(15,16,17,18,19) ORF plasmid. c Gel image of RT-qPCR product of circCCDC7(15,16,17,18,19) from 293 T cells transfected with vector, circCCDC7(15,16,17,18,19) overexpression or frame shift mutation group. d Western blot demonstrated the absence of FLAG tagged protein with the frame shift mutant. e Colony formation assay measuring cell viability in PC3 cells when different plasmids were transfected. f, g Representative images of migration and invasion assays using PC3 after different plasmids transfection. h, i Histogram analysis of migrated and invaded cell counts. j Representative image of the Western blotting analysis of Slug, E-cad, and Claudin protein levels after transfection with different plasmids in PC3 cells. The grayscale ratio of target protein to GAPDH was listed on top of the lanes. ***p < 0.001.