| Volume | |
|---|---|
| 2× Absolute qPCR SYBR Green Capillary Mix | 10 μL |
| 10 μM Forward primer | 0.4 μL |
| 10 μM Reverse primer | 0.4 μL |
| Template (cDNA) | 2 μL |
| Water, nuclease-free | 7.2 μL |
| Total volume | 20 μL |
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