Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2023 Aug 4;49(3):508–520. doi: 10.1038/s41386-023-01673-6

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© This is a U.S. Government work and not under copyright protection in the US; foreign copyright protection may apply 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Fig. 5 — A Experimental timeline of behavioral experiments. Male and female C57BL/6J mice were stereotaxically injected with AAV8-hSyn-DIO-hM3Dq into the right PBN and rAAV-hSyn-cre into the right CeA. Sciatic nerve surgery was performed 3 weeks after viral injections. Following 1 week of recovery, von Frey, Randall-Selitto, Acetone and Hargreaves tests were used to address sensitivity to tactile, deep tissue pressure, cold and heat stimulation, respectively. Mice were intraperitoneally (i.p.) injected with CNO and saline prior to behavior testing in a counterbalanced way. Paw withdrawal threshold after tactile (B, F) and pinch (C, G) stimulation, acetone response score (D, H) and withdrawal latency after heat stimulation (E, I) of the hind paw ipsilateral or contralateral to sham (B–E) and cuff (F–I) treatment before and 30 min after i.p. injection of CNO (green bar) or saline (gray bar). n = 7 mice per treatment for sham experiments (2 females and 5 males) and n = 6 mice per treatment for cuff experiments (1 female and 5 males). Two-way repeated measures ANOVA followed by Tukey’s multiple comparison test; ^####p < 0.0001 for before and after CNO in contralateral and ipsilateral paws to sham; ****p < 0.0001; **p < 0.01 for before and after CNO in contralateral paw to cuff. Blue (B–E) and red (F–I) symbols represent female mice and individual mice are represented by scatter points. All data are presented as means ± SEM.