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. 2024;27(2):157–164. doi: 10.22038/IJBMS.2023.72952.15863

Figure 2.

Effects of GABARAPL1 overexpression/knockdown in NPC 6-10B cell line

Figure 2

A. Overexpression or knockdown of GABARAPL1 was confirmed by Western blotting after 24 hr transfection. Grayscale analysis of western blot indicates the protein expression levels relative to GAPDH. B. EdU staining flow cytometry of NPC cells after overexpression or knockdown of GABARAPL1. The percentage of EdU-positive cells is shown. C. Apoptosis flow cytometry of NPC cells after overexpression or knockdown of GABARAPL1. Annexin V-FITC/PI was used. The percentage of late apoptotic cells is shown. Biological triplicates were performed. Ordinary one-way ANOVA and multiple comparisons were used for analysis. Data are presented as mean ± SD. ***P<0.001, **P<0.01, *P<0.05. Control, cells without interventions. Vector, cells transfected with empty vector. Lv_GABARAPL1, cells with overexpressed GABARAPL1. Sh_GABARAPL1, cells with knockdown of GABARAPL1.

GABARAPL1: gamma-aminobutyric acid (GABA) type A receptor-associated protein-like 1; NPC: nasopharyngeal carcinoma; GAPDH: glyceraldehyde 3-phosphate dehydrogenase; EdU: 5-ethynyl-2’-deoxyuridine; FITC: fluorescein isothiocyanate; PI: propidium iodide; ANOVA: analysis of variance; SD: standard deviation