Figure 3.

Effect of GABARAPL1 on HIF-2α

A. NPC 6-10B cell line-derived tumor growth in BALB/c nude mice. 6-10B cells with stable transfection of empty vector or GABARAPL1 were injected into nude mice (four mice/group). The tumor size and the tumor weight at the endpoint (15 days after cell injection) are shown. B. Representative images for H&E staining of tumor tissues from mice. C. Western blot analysis of GABARAPL1-related targets in the tumor tissues from mice at the endpoint. Grayscale analysis of western blot indicates the protein expression levels relative to GAPDH. Biological triplicates were performed. Data are presented as mean ± SD. Ordinary one-way ANOVA and multiple comparisons were used. ***P<0.001, **P<0.01, *P<0.05. Vector, tumors with empty vector. Lv_GABARAPL1, cells with overexpressed GABARAPL1.

GABARAPL1: gamma-aminobutyric acid (GABA) type A receptor-associated protein-like 1; HIF-2α: hypoxia-inducible factor 2 alpha; NPC: nasopharyngeal carcinoma; H&E: hematoxylin, and eosin; GAPDH: glyceraldehyde 3-phosphate dehydrogenase; SD: standard deviation; ANOVA: analysis of variance