Figure 4.
Effects of GABARAPL1 overexpression or knockdown on related genes in NPC 6-10B cell line
Representative image of western blotting and quantitative analysis of protein levels on ATG4B, LC3, P62, HIF-2α after overexpression or knockdown of GABARAPL1. Biological triplicates were performed. Data are presented as mean ± SD. One-way ANOVA and multiple comparisons were used for analysis. ***P<0.001, **P<0.01, *P<0.05. Control, cells without interventions. Vector, cells transfected with empty vector. Lv_GABARAPL1, cells with overexpressed GABARAPL1. Sh_GABARAPL1, cells with knockdown of GABARAPL1.
GABARAPL1: gamma-aminobutyric acid (GABA) type A receptor-associated protein-like 1; NPC: nasopharyngeal carcinoma; ATG4B: autophagy-related 4B cysteine peptidase; LC3: microtubule-associated protein 1 light chain 3; HIF-2α: hypoxia-inducible factor 2 alpha; SD: standard deviation; ANOVA: analysis of variance