Table 1.
Primer sequence for autophagy-related genes and internal reference
| Gene | Primer sequence | |
|---|---|---|
| GAPDH | F | TCAAGAAGGTGGTGAAGCAGG |
| R | GCGTCAAAGGTGGAGGAGTG | |
| ATG4B, mouse | F | TCAGGAAGTGGGTGTGTGGGAAA |
| R | GGCAATTCTCAGCAAGGCAAGGA | |
| LC3B, mouse | F | TTCAGGTTCACAAAACCCGC |
| R | TCTCACACAGCCCGTTTACC | |
| LC3A, mouse | F | GCCTTCTTCCTGCTGGTGAACC |
| R | TCCTCGTCTTTCTCCTGCTCGTAG | |
| HIF2α, mouse | F | CCGAACTGACCAGATATGACTGTGAG |
| R | AGTCTGCCAGGTAAGTCCATCTTGTA | |
| GABARAPL1, mouse | F | CGGAAGAGAATCCACCTGAGACCT |
| R | TCCAGTATTGTGCAACCAGAACCATT | |
| GABARAPL1, human | F | TCTCCATCTGGCTCTCCTCTACCT |
| R | TGGTCCTCCTTGTACTGGAACTTCAT | |
| p62, mouse | F | AGTCGGATAACTGTTCAGGAGGAGAT |
| R | AGCCAGCCGCCTTCATCAGA | |
GAPDH: glyceraldehyde 3-phosphate dehydrogenase; ATG4B: autophagy-related 4B cysteine peptidase; LC3A: microtubule-associated protein 1 light chain 3 alpha; LC3B, microtubule-associated protein 1 light chain 3 beta; HIF-2α: hypoxia-inducible factor 2 alpha; GABARAPL1: gamma-aminobutyric acid (GABA) type A receptor-associated protein-like 1