Table 2.
Antibody target | Dilution factor | Cat # and source |
---|---|---|
ATG4B | 1:1000 | 10482-1-AP, Proteintech |
GABARAPL1 | 1:1000 | 66575-1-Ig, Proteintech |
SQSTM1/P62 | 1:1000 | AF5384, Affinity |
HIF-2α | 1:500 | NB100-902HIF2, Novus |
LC3I/II | 1:500 | SAB3500350, Sigma-Aldrich |
GAPDH | 1:5000 | 10494-1-AP, Proteintech |
ATG4B: autophagy-related 4B cysteine peptidase; GABARAPL1: gamma-aminobutyric acid (GABA) type A receptor-associated protein-like 1; SQSTM1/P62: HIF-2α, hypoxia-inducible factor 2 alpha; LC3I/II: microtubule-associated protein 1 light chain 3 I/II; GAPDH: glyceraldehyde 3-phosphate dehydrogenase