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. 2024;27(2):157–164. doi: 10.22038/IJBMS.2023.72952.15863

Table 2.

Primer sequence for autophagy-related proteins and internal reference

Antibody target Dilution factor Cat # and source
ATG4B 1:1000 10482-1-AP, Proteintech
GABARAPL1 1:1000 66575-1-Ig, Proteintech
SQSTM1/P62 1:1000 AF5384, Affinity
HIF-2α 1:500 NB100-902HIF2, Novus
LC3I/II 1:500 SAB3500350, Sigma-Aldrich
GAPDH 1:5000 10494-1-AP, Proteintech

ATG4B: autophagy-related 4B cysteine peptidase; GABARAPL1: gamma-aminobutyric acid (GABA) type A receptor-associated protein-like 1; SQSTM1/P62: HIF-2α, hypoxia-inducible factor 2 alpha; LC3I/II: microtubule-associated protein 1 light chain 3 I/II; GAPDH: glyceraldehyde 3-phosphate dehydrogenase