FIG. 2.

Southern blotting performed with probes for hmw sequences and hia. Chromosomal DNA was digested with BglII, separated by agarose electrophoresis, and subjected to Southern analysis. (A) Southern analysis with a probe corresponding to the promoter and 5′ coding sequence of hmw1A. Strains by lane: 1, 12; 2, 11; 3, 3219B; 4, 3686; 5, 3242A; 6, 3219C; 7, 3655; 8, 1667; 9, 3246A; 10, 1484A; 11, 1674. Less chromosomal DNA was inadvertently loaded in lanes 7 and 9. (B) Southern analysis with a probe that represents an intragenic fragment of hia. Strains by lane: 1, 12; 2, 11; 3, 1862; 4, 3179B; 5, 3640; 6, 3A; 7, 1860; 8, 3230B; 9, 3248A; 10, 3230A; 11, 1396B. Comparable quantities of DNA were loaded in each lane.