Figure 2.
Effects of adenine acyclic bisphosphate derivatives on P2Y1 receptor-activated phospholipase C activity in turkey erythrocyte membranes: both concentration-dependent stimulation of inositol phosphate formation by 2-MeSADP (◯) and its inhibition in the presence of 30 nM 2-MeSADP by compounds 11 (●), 17 (■), and 18 (▲). Membranes from [3H]-inositol-labeled erythrocytes were incubated for 5 min at 30 °C in the presence of the indicated concentrations of 2-MeSADP or of test compound in combination with 30 nM 2-MeSADP. The data shown are typical curves for at least three experiments carried out in duplicate using different membrane preparations.