Figure 6.

Conformational dynamics of SPNS2 in the ternary complex with both S1P and PI(4,5)P₂.

(A) Heatmap representing ΔRFU as a function of time, comparing PI(4,5)P₂: S1P: SPNS2 with apo SPNS2.

(B) Deuterium uptake plot for peptide 259–263. Data are plotted as mean ± SD (n = 3).

(C) ΔRFU of PI(4,5)P₂: S1P: SPNS2 compared with apo SPNS2 mapped onto the predicted model of SPNS2. Ribbon representation is viewed from membrane.

(D) Deuterium uptake plot for peptide 470–478. Data are plotted as mean ± SD (n = 3).

(E) Deuterium uptake plot for peptide 117–126. Data are plotted as mean ± SD (n = 3).

(F) ΔRFU of PI(4,5)P₂: S1P: SPNS2 compared with apo SPNS2 mapped onto the predicted model of SPNS2. Ribbon representation is viewed from extracellular side.

(G) Deuterium uptake plot for peptide 336–340. Data are plotted as mean ± SD (n = 3).

(H–K) Changes in fractional deuterium uptake of peptides 117–126 (H), 259–263 (I), 336–340 (J), and 470–478 (K) for full-length and truncated SPNS2 at 60 min labeling. All data are plotted as mean ± SD (n = 3). ^∗p < 0.05. ^∗∗p < 0.01. ^∗∗∗p < 0.001. ^∗∗∗∗p < 0.0001.