Cellular functions of AIMP2. (A) CCK-8 assay results show a decrease in the viability of BT-549 and MDA-MB-231 cells upon AIMP2 knockdown (***, p < 0.001). (B) Transwell assay results show a decrease in the invasion ability in BT-549 and MDA-MB-231 cells upon AIMP2 knockdown (**, p < 0.01). (C) Scratch assay showed that AIMP2 knockdown decreased the migration ability of BT-549 and MDA-MB-231 cells. (D, E) Western blot and real-time quantitative PCR detection show the knockdown efficiency of AIMP2 (**, p < 0.01; ***, p < 0.001). (F) AIMP2 expression in normal and tumor tissues.