Key resources table

REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
Mouse anti-Aggrecan	Thermofisher	MA3-16888
Rabbit ant-βcatenin	Abcam	ab6302
Rabbit ant-βgalactosidase	MBL Life Science	PM049
Mouse anti-Collagen 2A1	Millipore	MAB8887
Rabbit anti-Osteocalcin	EMD Millipore	ab10911
Goat anti-Periostin	R&D Systems	af2955
Rabbit anti-Runx2	Abcam	ab23981
Rabbit anti-total βcatenin	Invitrogen	71-2700
Rabbit anti-non-phospho (active)-βcatein	Cell Signaling	8814
Rabbit anti-LRP5	Proteintech	24899-1-AP
Rabbit anti-LRP6	Cell Signaling	3395
Mouse anti-CD44	Proteintech	60224-1
Mouse anti-GAPH	Invitrogen	MA116757
Goat anti-mouse HRP	Invitrogen	G21040
Goat anti-rabbit HRP	Invitrogen	G21234
Goat anti-rabbit Alexa Fluor 546	Invitrogen	A11010
Goat anti-rabbit Alexa Fluor 488	Invitrogen	A11008
Goat anti-mouse Alexa Fluor 546	Invitrogen	A11003
Goat anti-mouse Alexa Fluor 488	Invitrogen	A11001
Donkey anti- rabbit Alexa Fluor 647	Invitrogen	A32795
Chemicals, peptides, and recombinant proteins
Recombinant Human Protein Sclerostin Protein	R&D Systems	1406-ST-025/CF
Sodium Hyaluronate	Lifecore Biomedical	HA2M-5
Recombinant Human Wnt3A Protein	R&D Systems	5036-WN-010/CF
Recombinant Human Dickkopf Related 3 Protein	R&D Systems	1118-DK-050/CF
Wnt-C59	Peprotech	1248913
Critical commercial assays
Porcine Dickkopf Related Protein 3 ELISA Kit	Mybioscience	MBS099882
Sclerostin Human ELISA Kit	Invitrogen	EHSOST
Invitrogen™ Click-iT™ Plus EdU Cell Proliferation Kit for Imaging	Invitrogen	C10639
SYBR™ Green PCR Master Mix	ThermoFisher	4309155
RNAscope™ Multiplex Fluorescent Reagent Kit v2	Advanced Cell Diagnostics	323100
RNAscope™ 2.5 HD Duplex Detection Reagents	Advanced Cell Diagnostics	322500
Deposited data
Raw and analyzed data	This paper	GEO: GSE236909
Experimental models: Primary Cell lines
1-Human Perichondrial Cells Lgr5High	Embree Lab, this paper	N/A
OA 2-Human Perichondrial Cells Lgr5Low	Embree Lab, this paper	N/A
OA 3-Human Perichondrial Cells Lgr5Low	Embree Lab, this paper	N/A
OA 4-Human Perichondrial Cells Lgr5Low	Embree Lab, this paper	N/A
Healthy Human Mandibular Condylar Chondrocytes	Embree Lab, this paper	N/A
Osteoarthrithic Human Mandibular Condylar Chondrocytes	Embree Lab, this paper	N/A
Mini-pig mandibular condylar chondrocytes	Embree Lab, this paper	N/A
Mini-pig perichondrial cells	Embree Lab, this paper	N/A
Experimental models: Organisms/strains
Mouse: prg4tm1Mawa/J	Jackson Laboratory	025737
Mouse: Tg (TCF/Lef1-lacZ)34Efu/J	Jackson Laboratory	004623
Mouse: Gt(ROSA)26Sortm9(CAG-tdTomato)Hze/J	Jackson Laboratory	007909
Mouse: Lgr5tm1(cre/ERT2)Cle/J	Jackson Laboratory	008874
Mouse: CD1-IGS	Charles River	022
Mouse: Lgr5DTR-EGFP	Genetech	N/A
Rat: Sprague Dawley	Charles River	400
Rabbit: New Zealand White	Charles River	052
Pig: Yucatan Miniature Swine	Sinclair Bioresources	N/A
Oligonucleotides
Primers for qRT-PCR, see Table S5	This paper	N/A
Primers for genotyping, see Table S2	This paper	N/A
Software and algorithms
Prism Graphpad	N/A	https://www.graphpad.com
ImageJ	Schneider et al. 2012	https://imagej.nih.gov/ij/
Biorender	N/A	https://www.biorender.com
StringTie software	Kovaka et al. 2019	https://ccb.jhu.edu/software/stringtie/
RUVSeq software	Risso et al. 2014	https://bioconductor.org/packages/release/bioc/html/RUVSeq.html
SQANTI3 software	Tardgauila et al. 2018	https://github.com/ConesaLab/SQANTI3
IsoformSwitchAnalyzeR software	Vitting-Seerup et al. 2019	https://bioconductor.org/packages/release/bioc/html/IsoformSwitchAnalyzeR.html
DESeq2 software	Love et al. 2014	https://bioconductor.org/packages/release/bioc/html/DESeq2.html
clusterProfiler 4.0 software	Wu et al. 2021	https://bioconductor.org/packages/release/bioc/html/clusterProfiler.html
Salmon aligner	Patro et al. 2017	https://combinelab.github.io/salmon/
STAR aligner	Dobin et al. 2013	https://github.com/alexdobin/STAR/tree/master