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. 2023 Nov 10;8(6):e00632-23. doi: 10.1128/msystems.00632-23

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Fig 3 — The C. tuberculostearicum pangenome. (a) Anvi’o pangenomic map for 28 C. tuberculostearicum genomes (including five NCBI reference genomes). Genomic rings are annotated by skin site and HV metadata and ordered by pyANI ANIb. Genome margins are manually adjusted for clarity. (b) Heap’s Law estimates of pangenome openness for C. tuberculostearicum complex (N = 28) and C. tuberculostearicum species (N = 20) genomes. Rarefaction curves show the total number of genes accumulated with the addition of new genome sequences in random order with 1,000 permutations. Shaded regions represent the 95% CI. A Heap’s law model was fit to the resultant complex and species curves to calculate γ values (0.30 ± 0.01 and 0.25 ± 0.01, respectively). (c) Number of core (belonging to all genomes), accessory (belonging to two or more genomes), and singleton (belonging to only one genome) genes. The expanded pangenome contains 5,451 genes using 90% sequence identity as a cutoff parameter.