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Example of three projects (Cldn10, Cotl1 and Isl1) analysed by Southern blot using radioactive probes. For each project, three or four clones have been processed. A lacZ probe was used only when no acceptable 5′ restriction size (> 20 kb) was possible (Cldn10 project). For the Cotl1 project, the 3′ ApaI digest for clone 3 does not show a double band, whilst other digests do. This suggests that two bands of the same size are generated by this enzyme.
