Fig 2.

Dexamethasone suppresses tumor initiation and growth of KS-like tumors. (A) Schematic illustration of experimental design. Stage I was designed to examine the effect of dexamethasone on tumor initiation and growth while stage II was designed to examine the effect of dexamethasone on grown tumors and maintenance of dexamethasone-treated tumors. Note that mice were inoculated on both flanks to generate two tumors per mouse. (B) Tumor incidence over time in vehicle- and dexamethasone-treated groups during stage I experiment. Each group contained 32 tumors from 16 mice. Log rank test was used for the trend analysis. (C) Tumor growth of vehicle- and dexamethasone-treated groups during stage I experiment. Each group contained 32 tumors from 16 mice. (D) Tumor growth of vehicle-treated mice from stage I experiment and further treated with either vehicle or dexamethasone in stage II experiment. Each group contained 16 tumors from 8 mice. (E) Tumor growth of dexamethasone-treated mice from stage I experiment and further treated with either vehicle or dexamethasone in stage II experiment. Each group contained 16 tumors from 8 mice. (F) Ki67 staining of the KS-like tumors. Scale bar: 50 µm. (G) Dual immunofluorescence staining of LANA and GR or LANA and pGR-S211 of the KS-like tumors. Scale bar: 20 µm. Student’s t test was used for the analysis, and results are presented as mean + 95% CI (C–E).