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. 2023 Dec 20;15(1):e03011-23. doi: 10.1128/mbio.03011-23

Fig 4.

Fig 4

IL-1α mediates dexamethasone suppression of cell proliferation in KSHV-transformed cells. (A and B) IL-1α mRNA expression in MM and KMM cells treated with 0 or 0.1 µM dexamethasone measured by RNA-seq (A) and RT-qPCR (B). (C) IL-1α protein level in MM and KMM cells treated with 0 or 0.1 µM dexamethasone for 2 days detected by ELISA. (D) IL-1α mRNA expression in MM and KMM cells following knockdown of GR treated with 0 or 0.1 µM dexamethasone for 2 days measured by RT-qPCR. Cells were treated with dexamethasone 1 day after siRNA transfection. (E) IL-1α protein level in MM and KMM cells following knockdown of GR treated with 0 or 0.1 µM dexamethasone for 2 days detected by ELISA. (F and G) Cell proliferation curves of KMM (F) and MM (G) cells treated with 0 or 0.1 µM dexamethasone and 0.1, 1, 10, or 100 ng/mL recombinant IL-1α protein. (H and I) Dual-immunofluorescence staining of LANA and IL-1α in human KS tumors (H) or vehicle- and dexamethasone-treated mouse KS-like tumors (I). Scale bar: 20 µm. Student’s t test was used for the analysis and results from three independent repeats are presented as mean ± SD (B, D, F, and G).