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. 2023 Dec 20;15(1):e03011-23. doi: 10.1128/mbio.03011-23

Fig 5.

Fig 5

Induction of IL-1Ra marginally mediates dexamethasone suppression of cell proliferation in KSHV-transformed cells. (A and B) IL-1Ra mRNA expression in MM and KMM cells treated with 0 or 0.1 µM dexamethasone for 2 days measured by RNA-seq (A) and RT-qPCR (B). (C) IL-1Ra mRNA expression in MM and KMM cells following knockdown of GR treated with 0 or 0.1 µM dexamethasone measured by RT-qPCR. Cells were treated with dexamethasone 1 day after siRNA transfection. (D) Schematic illustration of the differences between rat natural IL-1Ra protein and predicted variants X1 and X2. (E and F) Natural IL-1Ra, predicted X1 and X2 mRNA (E), and protein (F) levels in overexpression KMM cells measured by RT-qPCR and ELISA, respectively. (G) Cell proliferation curves of KMM cells overexpressing IL-1Ra and variants described in panel D. (H and I) Analysis of cell cycle (H) and apoptosis (I) of KMM cells overexpressing IL-1Ra and variants described in panel D. Student’s t test was used for the analysis, and results from three independent repeats are presented as mean ± SD (B, C, and E–I).