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. 2023 Dec 20;15(1):e03011-23. doi: 10.1128/mbio.03011-23

Fig 7.

Fig 7

vFLIP inhibition of the GR pathway in KSHV-transformed cells does not impede cell responsiveness to dexamethasone. (A) GR mRNA expression levels in MM and KMM cells or cells harboring ΔmiRNA, ΔvFLIP, or ΔvCyclin mutant. (B) Immunoblots of GR and pGR-S211 in MM and KMM cells or cells harboring ΔmiRNA, ΔvFLIP, or ΔvCyclin mutant. (C) GR mRNA expression levels in MM cells expressing the miRNA cluster or the vector control. (D) Immunoblots of GR and pGR-S211 in MM cells expressing the miRNA cluster or the vector control. (E) GR mRNA expression levels in MM cells expressing vFLIP, vCyclin, or the vector control. (F) Immunoblots of GR and pGR-S211 in MM cells expressing vFLIP, vCyclin, or the vector control. (G) GR mRNA expression levels in MM and KMM cells stably expressing IκBαM or the vector control. (H) Immunoblots of GR and pGR-S211 in MM and KMM cells stably expressing IκBαM or the vector control. (I–K) Proliferation curves of KMM cells and cells harboring ΔmiRNA, ΔvFLIP, or ΔvCyclin mutant (I), MM cells expressing the miRNA cluster or the vector control (J), or MM cells overexpressing vFLIP, vCyclin, or the vector control (K) treated with 0 or 0.1 µM dexamethasone. (L and M) IL-1α mRNA (L) and protein (M) levels in KMM cells and cells harboring ΔmiRNA, ΔvFLIP, or ΔvCyclin mutant treated with 0 or 0.1 µM dexamethasone for 2 days measured by RT-qPCR and ELISA, respectively. (N and O) IL-1α mRNA (N) and protein (O) levels in MM cells expressing the miRNA cluster or vector control treated with 0 or 0.1 µM dexamethasone for 2 days measured by RT-qPCR and ELISA, respectively. (P and Q) IL-1α mRNA (P) and protein (Q) levels in MM cells expressing vFLIP, vCyclin, or vector control treated with 0 or 0.1 µM dexamethasone for 2 days measured by RT-qPCR and ELISA, respectively. (R–T) IL-1Ra mRNA expression levels in KMM cells and cells harboring ΔmiRNA, ΔvFLIP, or ΔvCyclin mutant (R), MM cells expressing the miRNA cluster or the vector control (S), or vFLIP, vCyclin or the vector control (T) treated with 0 or 0.1 µM dexamethasone for 2 days. Student’s t test was used for the analysis and results from three independent repeats are presented as mean ± SD (A, C, E, G, I–L, N, P, and R–T).