TABLE 2.
N-termini of known PNEPs and the SURFIN familyb
| Gene ID | Protein name (former ID) | N-terminus | Methionine amino peptidase | New N-terminus | |
|---|---|---|---|---|---|
| PF3D7_0501300 | SBP1 | MCSAARAFDFFTDLA | ✓ | C | |
| PF3D7_1370300 | MAHRP1 | MAEQAAVQPESVPTV | ✓ | A | |
| PF3D7_1353200 | MAHRP2 | MQPCPYDVYNQINHV | ✗ | Q | |
| PF3D7_0935900 | REX1 | MADYSSNEEETPKEE | ✓ | A | |
| PF3D7_0936000 | REX2a | MKMYLAEIFSSGKES | ✗ | K? | Y? |
| PF3D7_1149000 | Pf332 | MCSAARAFDFFTDLA | ✓ | C | |
| PF3D7_0702400 | SEMP1/PF07_0007 | MSQPQKQQNEEGAAT | ✓ | S | |
| PF3D7_0702500 | PF07_0008 | MAYPLLEDDLRSIRV | ✓ | A | |
| PF3D7_1334700 | MSP7-like/PF13_0194 | Signal sequence predicted 27 and 28: SQS-VN | NA | V | |
| PF3D7_1404800 | PF14_0045 | Signal sequence predicted 21 and 22: TWA-LF | NA | L | |
| PF3D7_0830500 | TryThrA/PF08_0003 | MNLEQFKNINKDLAT | ✗ | N | |
| PF3D7_1148900 | PF11_0505 | MEAEKKEEKQEKSVK | ✗ | E | |
| PF3D7_0113100 | SURFIN 1.1 | MEQIGIANNIFTEIG | ✗ | E | |
| PF3D7_0113600 | SURFIN 1.2 | MHFVVEFQDWIKEPK | ✗ | H | |
| PF3D7_0115000 | SURFIN 1.3 | MAVKISTSVYKKRIS | ✓ | A | |
| PF3D7_0402200 | SURFIN 4.1 | MHFVVELDNTGDDF | ✗ | H | |
| PF3D7_0424400 | SURFIN 4.2 | MLFVVELDSRLEKSAD | ✗ | L | |
| PF3D7_0831100 | SURFIN 8.1 | MAIISSIPNYRKGLNP | ✓ | A | |
| PF3D7_0830800 | SURFIN 8.2 | MLMVIEFDDPIKEPS | ✗ | L | |
| PF3D7_0800700 | SURFIN 8.3 | MAVEMNTPVSKKGVS | ✓ | A | |
| PF3D7_1301800 | SURFIN 13.1 | MASYIKLNIPLKPKK | ✓ | A | |
| PF3D7_1477600 | SURFIN 14.1 | MKVKKDNPNYKKRIN | ✗ | K |
a
Experimental verification of the N-terminus of REX2 revealed the presence of peptides starting with an A or L.
b
Indicated are the N-termini of the protein, whether the start methionine is likely to be removed and the expected N-terminal residue after processing by methionine aminopeptidase. NA, not applicable.