Fig 7.

Lyophilized mLNP-circRNA-G remains a target for delivery and potent immunogenicity. (A) Lyophilized mLNP-circRNA-Luc was stored at 4 ℃ for 24 weeks. Fluorescence images of DiR-labeled mLNP-circRNA-Luc (containing 5 µg circRNA) 24 h after intramuscular injection (n = 3). (B) Bioluminescence imaging of mice 24 h after intramuscular injection with freshly prepared or lyophilized mLNP-circRNA-Luc. (C–F) Mice were inoculated with a single dose of 2 µg of freshly prepared or lyophilized mLNP-circRNA-G by intramuscular injection, and the IgG titers (C and E) and nAb titers (D and F) of serum harvested in week 2 and week 4 were measured. (G and H) Survival rates (G) and body weight changes (H) of mice. (I and J) Histological and viral level analysis of brain sections. Representative images of RABV detected using RABV P protein monoclonal antibody (I). Brains were sectioned and stained with H&E (J).