TABLE 2.
Bacterial strains used in this study
| Strain | Genotype | Source/reference |
|---|---|---|
| E. coli strains | ||
| DH5α | E. coli supE44 ∆lacU169 (φ80lacZΔM15) hsdR17 recA1 endA1 gyrA96 thi-1 relA1 | Invitrogen |
| DH5α/pRK212.1 | DH5α with conjugation transfer element | (114) |
| Top10 | F− mcrA ∆(mrr-hsdRMS-mcrBC) φ80lacZΔM15 ∆lacX74 recA1 araD139 ∆(ara-leu)7697 galE15 galK16 rpsL (Strr) endA1 nupG | Invitrogen |
| XL1-Blue | F′::Tn10 proA+B+lacIqΔ(lacZ)M15/recA1 endA1 gyrA96 (Nalr) thi hsdR17 (rK− mK+) glnV44 relA1 lac | New England Biolabs |
| Campylobacter jejuni strains | ||
| DRH212 | 81–176 rpsLSm | (115) |
| DRH837 | 81–176 rpsLSm/pECO102 | (116) |
| ABT501 | 81–176 rpsLSmΔvidA | (88) |
| DAR5362 | 81–176 rpsLSm ΔvidA/pECO102 | This study |
| DAR5364 | 81–176 rpsLSmΔvidA/pDAR5476 | This study |
| DAR5615 | 81–176 rpsLSmΔvidA/pDAR5357 | This study |
| DAR6160 | 81–176 rpsLSm cbrR::cat-rpsL | This study |
| DAR6162 | 81–176 rpsLSmΔvidA cbrR::cat-rpsL | This study |
| DAR6236 | 81–176 rpsLSmΔvidA vidB::cat-rpsL | This study |
| DAR6232 | 81–176 rpsLSm vidB::cat-rpsL | This study |
| DAR6249 | 81–176 rpsLSmΔcbrR | This study |
| DAR6307 | 81–176 rpsLSmΔvidAΔcbrR | This study |
| DAR6357 | 81–176 rpsLSmΔvidA ΔvidB | This study |
| DAR6668 | 81–176 rpsLSmΔvidA vidBS363G | This study |
| DAR6408 | 81–176 rpsLSmΔvidB | This study |
| DAR6672 | 81–176 rpsLSm vidBS363G | This study |
| DAR6673 | 81–176 rpsLSm/pDAR1604 | This study |
| DAR6675 | 81–176 rpsLSmΔvidA/pDAR1604 | This study |
| DAR6727 | 81–176 rpsLSm/pDAR6580 | This study |
| DAR6812 | 81–176 rpsLSmΔvidA/pDAR6606 | This study |
| DAR6851 | 81–176 rpsLSm/pDAR6301 | This study |
| DAR6853 | 81–176 rpsLSmΔvidA/DAR6301 | This study |