TABLE 3.
Plasmids used in this study
| Plasmid | Genotype | Source/reference |
|---|---|---|
| pUC19 | AmpR, general cloning vector | New England BioLabs |
| pQE30 | AmpR, expression vector for adding 6XHis-tag to N-terminus of proteins | Qiagen |
| pBAD/myc-HisA | Invitrogen | |
| pRY108 | KanR, E. coli-C. jejuni shuttle vector | (117) |
| pRY109 | Source of cat cassette | (117) |
| pDRH265 | Source of cat-rpsL cassette | (115) |
| pECO102 | CatR, E. coli-C. jejuni shuttle vector containing cat promoter and stop codon for expression of genes for complementation | Wiesner |
| pVL791 | Contains wspR | (114) |
| pDAR964 | CatR, E. coli-C. jejuni shuttle vector containing cat promoter with an in-frame N-terminal FLAG sequence for expression of genes for complementation | (118) |
| pDAR1425 | pRY108 with 206 bases of DNA containing promoter for flaA and start codon cloned into the XbaI and BamHI sites | This study |
| pDAR1604 | pRY108 with 206 bases of DNA containing promoter for flaA and sequence for FLAG tag fused in-frame to the start codon cloned into the XbaI and BamHI sites | (119) |
| pDAR5357 | pDAR964 with codon 2 to stop codon of vidA cloned into the BamHI site of pDAR964 to create a N-terminal FLAG tag fusion protein | This study |
| pDAR5476 | pRY112 with 76 bases of DNA containing promoter for cat and start codon with in-frame BamHI site, FLAG tag epitope sequence, and stop codon cloned into the XbaI and PstI sites for expression of C-terminal FLAG-tagged proteins for complementation | This study |
| pDAR5571 | pDAR5476 with codon 2 to penultimate codon of vidA cloned in-frame in BamHI site | This study |
| pDAR6131 | pUC19 with DNA fragment to create in-frame deletion of codons 47–347 of vidB with 0.8 kb of upstream and downstream sequences cloned in to the EcoRI site | This study |
| pDAR6134 | pQE30 with codon 2 to stop codon of vidB cloned into the BamHI site | This study |
| pDAR6144 | pUC19 with DNA fragment to create in-frame deletion of codon 2 to 375 of cbrR with 0.8 kb of upstream and downstream sequences cloned in to the EcoRI site | This study |
| pDAR6141 | pUC19 with DNA fragment containing cbrR with 0.8 kb of upstream and downstream sequences cloned into the EcoRI site; contains G522A mutation to create an EcoRV site within cbrR | This study |
| pDAR6152 | SmaI cat-rpsL cassette cloned into the EcoRV site of cbrR in pDAR6141 | This study |
| pDAR6158 | pUC19 with DNA fragment containing vidB with 0.8 kb of upstream and downstream sequences cloned into the EcoRI site, contains C890A mutation to create an EcoRV site within vidB | This study |
| pDAR6203 | pBAD/myc-HisA with codon 2 to penultimate codon of cbrR cloned into the NcoI and HindIII sites | This study |
| pDAR6214 | SmaI cat-rpsL cassette cloned into the EcoRV site of vidB in pDAR6158 | This study |
| pDAR6301 | pDAR1425 with cbrR from codon 2 to the stop codon cloned into the BamHI site | This study |
| pDAR6535 | pBAD/myc-HisA with codon 2 to penultimate codon of wspR cloned into the NcoI and HindIII sites | This study |
| pDAR6574 | pUC19 with DNA fragment containing vidBS363G with 0.8 kb of upstream and downstream sequence cloned into the EcoRI site | This study |
| pDAR6580 | pDAR1425 with vidB from codon 2 to the stop codon cloned into the BamHI site | This study |
| pDAR6606 | pDAR1425 with vidBS363G from codon 2 to the stop codon cloned into the BamHI siteco | This study |