Fig 1.
The effect of IL-22 deficiency on the spreading of pGP3-deficient C. muridarum from the small intestine into the large intestine. C57BL/6J mice without (panel a, n = 4) or with deficiency in IL-22 (b, IL-22−/−, n = 4) were inoculated with 1 × 105 inclusion forming units of pGP3-deficient C. muridarum (CMpGPG3S) via intrajejunal injection. Three days after the inoculation, mice were sacrificed for collecting stomach (Sto), small intestine (SI) tissues [duodenum (Duo), jejunum (Jej), and ileum (Ile)], and large intestine (LI) tissues [Cecum (Cec), colon (Col) and rectum (Rec)] as listed along the X-axis. Live CMpGP3S organisms were recovered from each tissue sample and expressed as log10 IFUs per tissue. The data came from two independent experiments. Note that significant levels of live chlamydial organisms were detected in the large intestine of IL-22−/− mice. * denotes an observed P-value <0.05, based on a Wilcoxon rank-sum test, between C57 and IL-22−/− in IFU recovery from the overall large intestinal tissues.