Fig 2.

Dead or inactivated N. gonorrhoeae do not inhibit C. trachomatis EB titer during co-infection. (A) N. gonorrhoeae (Ng) CFU/mL after 12 hours of co-infection. The dotted line denotes the limit of detection for this experiment. (B) Quantitative PCR analysis of genome copy number for C. trachomatis (EBs, RBs, and ABs) after 24 hours of growth in HeLa cells including 12 hours of co-infection with treated or untreated N. gonorrhoeae. (C) Quantitative immunofluorescence analysis of C. trachomatis D (CtD) EB titer after 24 hours of growth in HeLa cells including 12 hours of co-infection with treated or untreated N. gonorrhoeae. (C). Statistical analysis for data (C) is by one-way ANOVA P = 0.0014, with Tukey’s repeated measures for the post hoc test, *P < .05. Error bars are SEM and each experiment was performed in triplicate, three or four times.