Fig 5.

Supplementation of co-infection with 10 μM indole or 400 μM tryptophan does not restore the elementary body (EB) titer to mono-infection levels. HeLa cells were pre-infected with C. trachomatis CtD and then challenged with N. gonorrhoeae (Ng) with or without tryptophan or indole for 12 hours of growth. (A) N. gonorrhoeae (Ng) CFU/mL after 12 hours of co-infection with C. trachomatis D ± tryptophan or indole. (B) Quantitative PCR measurement of genome copy number for all forms of C. trachomatis (CtD) (EBs, RBs, and ABs) after 24 hours of growth in HeLa cells including 12 hours of co-infection with or without N. gonorrhoeae ± tryptophan or indole. (C) Quantitative immunofluorescence analysis of C. trachomatis D (CtD) EB titer after 24 hours of growth in HeLa cells including 12 hours of co-infection with or without N. gonorrhoeae ± tryptophan or indole. Each experiment was performed with three technical replicates and each experiment was performed three times. Error bars are the standard error of the mean (SEM) and * represents P < 0.05 (Tukey’s multiple comparisons test). Results are significantly different by one-way ANOVA (P < 0.01).