FIG. 1.
Schematic diagrams of P. falciparum RAP-1, the recombinant protein A-2-2, and recombinant fusion proteins MBP-RAP-11–294, p82T1.24, p82T1.24ΔHindIII, p82T1.24del6, and p82T1.24del11. The locations of structural features of RAP-1 are indicated (including those of the octameric repeats, iB-1, iB-2, and the Cys-containing region). Within the recombinant constructs, the termini of the RAP-1 fragments, the approximate lengths of the fusion partners MBP and trpE, and the locations of the 6-His residues are provided. The locations of BamHI (B) and HindIII (H) restriction sites are shown; a second HindIII site, located in the vector DNA of the p82T1.24 construct and used to construct p82T1.24ΔHindIII, is not shown. The RAP-11–294 fragment is found in A-2-2, MBP-RAP-11–294, and p82T1.24.