FIG. 3.

The binding of owl monkey antibodies to the recombinant RAP-1 fragment A-2-2, the peptide iB-1, and the unrelated peptide Ova_323–339. (A) Scatter plots of antibody reactivities to the three antigens. The means of triplicate ELISA determinations of normal monkey serum (⧫), serum after the first infection with P. falciparum ( ), and monkey serum after a second infection (▴) (diluted 1:1,000) on the recombinant and peptide antigens are shown. The peptide FL-160#3 (not shown) produced results nearly identical to those of Ova_323–339. (B) Comparison of anti-A-2-2 and anti-iB-1 antibody responses in individual monkeys. Shown are reactivities of individual monkey sera, obtained after a second P. falciparum infection, to recombinant protein A-2-2 (black) and peptide iB-1 (white). Serum samples 1 to 6 are from monkeys AI1707, AI1708, AI1709, AI1711, AI1447, and AO510, respectively. pep, peptide.