FIG. 7.

Amino acid sequence in the region of the N terminus of p67 including the locations of iB-1 and iB-2. An alignment of deduced amino acid sequences of the RAP-1 gene from P. falciparum laboratory isolates (Sierra Leone, Tanzania I/CDC, K1, and Honduras I/CDC) and the wild-type isolate India D (GenBank accession numbers L10322, L10323, M32853, M80807, and U41074, respectively) is given (11, 12, 27). The locations of the iB-1 and iB-2 epitopes are underlined, and the cleavage site responsible for the formation of p67 from the p82 precursor is marked (25). Sites of amino acid differences in this region are highlighted in boldface. Both Ala and Val were observed at X (position 184) in different genetic clones derived from the Sierra Leone isolate.