Figure 3.

The four TSPs of AV101 recognize different O-antigens of ESBL E. coli. (A) Phage AV101 infection on different bacterial hosts showing diverse plaque morphologies. (B) Successful phage infection or detection of a clear translucent zone on bacterial lawns are indicated with plus sign. TSP3 makes small translucent zones on two E. coli strains expressing the O8 O-antigen (+). Big clear translucent zone is indicated with a + sign. Small translucent zone is indicated with (+) sign. (C) Inhibition of AV101 infectivity on ESBL E. coli host after individual TSPs was preincubated with the ESBL hosts. TSP1 and TPS2 were able to completely block the infection of AV101 on their respective hosts, whereas only a partial inhibition with TSP3 and TSP4 could be observed.