Table 1.

Overview of primers for cloning of the four tsp genes.

Primers	Sequences (5’–3’)
AV101_TSP1_F	GCAAGCTTGTCGACGGAGTTACTGGATAACAATACTACACTCCATATCTGAAACAAC
AV101_TSP1_R	CGCGGATCCGAATTCGAGATGAACGAAATGTTCTCACAAGGTGGTAAAG
AV101_TSP2_F	GCAAGCTTGTCGACGGAGTTAATTACTAATCTTACCACGTATGGATAGCTCAAGAGC
AV101_TSP2_R	CGCGGATCCGAATTCGAGATGACCAGAAACGTCGAGAGCATC
AV101_TSP3_F	GCAAGCTTGTCGACGGAGTTATAGCTGGCAAGTAAAGCTCAAGTCTATTGATGC
AV101_TSP3_R	CGCGGATCCGAATTCGAGATGATTTCTCAATTCAATCAACCACGCGG
AV101_TSP4_F	GCAAGCTTGTCGACGGAGTTATATTACTGACGTCACAGTTCCAGATATAGACGC
AV101_TSP4_R	CGCGGATCCGAATTCGAGATGGCTAATAAACCAACACAGCCTGTTTTC
pET-28a(+)_F	CTCGAATTCGGATCCGCG
pET-28a(+)_R	CTCCGTCGACAAGCTTGC