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. 2023 Dec 19;100(1):fiad164. doi: 10.1093/femsec/fiad164

Figure 1.

Figure 1.

Timeline and experimental set-up of the great tit study. Generally, five microbiota samples were collected per nest: E0-egg—egg content sampled on embryonic day 0 (E0), E13-nat—E13 intestinal sample from a nonmanipulated egg, E13-Ent—E13 intestinal sample from an Enterococcus-treated egg, E13-PBS—E13 intestinal sample from a control, PBS-injected egg, and F—female faecal samples collected between days 7 and 15 (D1–D15) of nestling age. E and D above the axis indicate the day of embryonic and chick development, respectively.