Details of all the independent variables generated in the CCD and their effect (responses) on the CQAs of the optimized formulation.

Std	Run	Factor 1	Factor 2	Factor 3	Response 1	Response 2	Response 3	Response 4	Response 5	Response 6
		A: PLGA Polymer (mg)	B: PF127 (%)	C: MTF and MI (mg) same quantity for individual drugs	Particle size of MTF (D1) (nm)	Particle size of MI (D2) (nm)	PDI of MTF (D1)	PDI of MI (D2)	% EE of MTF (D1)	% EE of MI (D2)
7	1	20	0.6	60	187.2	188.2	0.116	0.099	50.46	52.12
6	2	40	0.2	60	254.4	256.12	0.389	0.381	67.14	65.85
8	3	40	0.6	60	247.7	248.2	0.234	0.201	64.45	65.12
4	4	40	0.6	40	245.6	247.6	0.136	0.231	68.29	69.01
9	5	13.18	0.4	50	175.8	176.4	0.182	0.208	44.89	49.5
10	6	46.82	0.4	50	289.6	288.5	0.355	0.343	68.57	69.97
14	7	30	0.4	66.82	196.8	197.4	0.159	0.15	57.85	58.49
13	8	30	0.4	33.18	195.8	196.9	0.173	0.201	65.14	66.13
11	9	30	0.04	50	235	221.7	0.346	0.356	65.23	65.21
1	10	20	0.2	40	185.5	184.9	0.261	0.261	61.61	58.26
12	11	30	0.74	50	200.9	188.9	0.146	0.164	61.95	59.87
18	12	30	0.4	50	195	198.5	0.17	0.153	66.25	67.13
19	13	30	0.2	50	205	206.8	0.26	0.248	66.98	66.25
5	14	20	0.2	60	192.6	194.2	0.145	0.231	53.98	56.25
3	15	20	0.6	40	181.9	180.8	0.201	0.241	56.74	56.03
2	16	40	0.2	40	254.2	252.8	0.34	0.312	69.84	68.91
15	17	30	0.6	50	194.9	196.5	0.141	0.145	63.85	63.74
20	18	30	0.4	60	210.5	220	0.168	0.164	62.22	64.11
16	19	30	0.4	40	195.8	197.2	0.175	0.175	66.18	66.92
17	20	30	0.6	60	202.8	203.4	0.14	0.094	60.48	60.23