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. 2024 Jan 17;291(2015):20232578. doi: 10.1098/rspb.2023.2578

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© 2024 The Authors.

Published by the Royal Society under the terms of the Creative Commons Attribution License http://creativecommons.org/licenses/by/4.0/, which permits unrestricted use, provided the original author and source are credited.

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Figure 3. — Expression of olfactory receptors in the female antenna. Fluorescent RNA in situ hybridization (FISH) and fluorescent immunohistochemistry (FIHC) alone or in combination. Fluorescence channels overlaid with the transmitted light channel. Scale bars, 20 µm. (a) FIHC with anti-ORco antibody [34] (magenta) labelling somata and dendrites of OSNs that innervate medium trichoid (mt), long trichoid (lt) and basiconic (b) sensilla. (b) FISH with Or19-specific RNA probe (magenta) combined in (b’,b’’) with FIHC using anti-HRP antibody (green, neuronal marker), showing innervation (arrowheads) of long trichoids (b’) and medium trichoids (b’’) by Or19-expressing neurons (encircled somata). (c) FISH with Ir8a-specific RNA probe (magenta) combined in (c’) with FIHC using anti-HRP antibody (green), showing innervation (arrowheads) of a long trichoid by an Ir8a-expressing neuron (encircled soma). (d) FISH with Ir8a-specific RNA probe (magenta) combined with FIHC using anti-ORco antibody (green). Encircled, Ir8a-positive somata; arrowheads, ORco-positive somata.