Figure S5.

Microglial and astrocytic recruitment to plaques during acute and washout phase. (A) Representative image of staining of X34⁺ plaques and P2RY12⁺ microglia following two doses of IgG control or aducanumab. Scale bar = 50 µm. (B and C) Representative image of staining of X34⁺ plaques and LGALS3⁺ microglia during (B) acute treatment phase after four doses, and (C) washout phase, 15 wk after four doses. Scale bar = 100 µm. (D) Representative image of staining of activated CD68⁺ microglia around X34⁺ plaques following two doses of IgG or aducanumab. Scale bar = 20 µm. (E) Quantification of total mean intensity of LGALS3⁺ microglia after two doses. (F) Quantification from Sholl analysis of intensity of LGALS3⁺ microglia 5 µm from plaques after two doses. (G) Quantification of total mean intensity of LGALS3⁺ microglia at 30 wk washout. (H) Quantification from Sholl analysis of intensity of LGALS3⁺ microglia 5 µm from plaques at 30 wk washout. (I) Quantification of total mean intensity of CD68⁺ microglia after two doses. (J) Quantification from Sholl analysis of intensity of CD68⁺ microglia 5 µm from plaques after two doses. (K) Quantification of total mean intensity of CD68⁺ microglia after four doses. (L) Quantification from Sholl analysis of intensity of CD68⁺ microglia 5 µm from plaques after four doses. (M) Quantification of total mean intensity of CD68⁺ microglia at 15 wk washout. (N) Quantification from Sholl analysis of intensity of CD68⁺ microglia 5 µm from plaques at 15 wk washout. (O) Quantification of total mean intensity of CD68⁺ microglia at 30 wk washout. (P) Quantification from Sholl analysis of intensity of CD68⁺ microglia 5 µm from plaques at 30 wk washout. (Q) Quantification of total coverage of GFAP⁺ astrocytes after four doses. (R) Quantification from Sholl analysis of coverage of GFAP⁺ astrocytes 15 µm from plaques after four doses. (S) Quantification of total coverage of GFAP⁺ astrocytes at 30 wk washout. (T) Quantification from Sholl analysis of coverage of GFAP⁺ astrocytes 15 µm from plaques at 30 wk washout. *P < 0.05; **P < 0.01, Student’s t test for normally distributed samples with no significant difference in variance (F females; G and H; I females; J–M; N males; O; P females; Q; R females; S; T males), Welch’s t test for normally distributed samples with differences in variances (F males; N females; P males; R males; T females), Mann–Whitney test for non-normally distributed samples (E; I males), with males and females analyzed separately. N = 4–11 mice/sex/treatment.