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. 2023 Mar 30;42(1):65–71. doi: 10.1038/s41587-023-01717-8

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Extended Data Fig. 2 — Shown are XYZ maximum projections of example PSFs taken at different FOV positions in different immersion media ranging from air and water to fused silica matching oil (FS Oil, Cargille 50350), and Type A oil (Cargille 16482). In general, the PSF size decreases with index as the increased NA leads to higher resolution. However, beyond n = 1.45, there is no change in resolution. We attribute this to residual aberrations from the minimally refractive inner surface of the correction plate: As the correction plate is made of fused silica with n = 1.45, manufacturing-related surface irregularities have no effect at this index. Scale bar: 1 µm.