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. 2024 Jan 16;10:33. doi: 10.1038/s41420-024-01808-8

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© The Author(s) 2024

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — A The protein expression of HOOK3 was examined in MKN-28 and HGC-27 cells with stable overexpression of HOOK3. GAPDH was used as a loading control. The quantification of band densities in Western blot analysis was performed using the ImageJ program. B The proliferation of MKN-28 and HGC-27 cells with stable overexpression of HOOK3 was assessed using a CCK-8 assay. C The colony formation assay was conducted to evaluate the ability of HOOK3 overexpressing MKN-28 and HGC-27 cells to form colonies. D EdU analysis was carried out to measure the proliferative ability of MKN-28 and HGC-27 cells with HOOK3 overexpression. Representative images are presented, with a scale bar of 100 μm. The percentage of EdU-positive cells in transfected GC cells was statistically analyzed and shown in the bar graph. E Transwell migration and invasion assays were performed to examine the migratory and invasive capacities of MKN-28 and HGC-27 cells with HOOK3 overexpression. F Apoptosis assays were performed on MKN-28 and HGC-27 cells with HOOK3 overexpression. The experiments were conducted in triplicate. The data were represented as means with standard deviation (SD), and statistical significance was assessed using Student’s t-test. **P < 0.01, and ***P < 0.001.