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. 2024 Jan 16;15:546. doi: 10.1038/s41467-024-44808-z

Fig. 2. Increased mtDNA mutation burden results in NAD+ depletion during the intestinal aging.

Fig. 2

a Gene Ontology (GO) enrichment of differentially upregulated genes in Mut/Mut*** mice at 8 months of age versus WT/WT* mice was performed using clusterProfiler package, and significance was determined by Fisher exact test of adjusted p-value by Benjamini–Hochberg (BH) method (n = 3 mice per group). b Detection of NADH/NAD+ ratio in the intestinal crypts of Mut/Mut*** mice and WT/WT* mice at 8 months of age using SoNar and cpYFP imaging (Scale bar, 10 μm). c Quantification of NADH/NAD+ ratios as in (b) (90 intestinal crypts from 3 mice per group; unpaired comparison using nonparametric Mann–Whitney test). Central line: median, box: interquartile ranges (IQR), whisker: ranges except extreme outliers (>1.5*IQR), circles: individual data points; d Detection of NAD+ content in the intestinal crypts of Mut/Mut*** mice and WT/WT* mice using mCherry-FiNad and mCherry-cpYFP imaging (Scale bar, 10 μm). e Quantification of mCherry-FiNad fluorescence corrected by mCherry-cpYFP as in (d) (90 intestinal crypts from 3 mice per group; unpaired comparison using nonparametric Mann–Whitney test). Central line: median, box: IQR, whisker: ranges except extreme outliers (>1.5*IQR), circles: individual data points. Source data are provided with this paper.