Skip to main content
. 2024 Jan 16;15:546. doi: 10.1038/s41467-024-44808-z

Fig. 3. NAD+ repletion alleviates small intestine aging phenotypes caused by mtDNA mutation burden.

Fig. 3

a Representative images of small intestine stained with H&E in Mut/Mut*** mice at 8 months of age with NMN or control (water) (Scale bar, 100 μm). Villus length and number of crypts per millimeter are quantified on right; WT/WT* is used as an additional control (Data are presented as the mean ± S.D of 9 intestinal segments from 3 mice per group; unpaired two-tailed Student’s t-test). b Representative images of intestinal crypts stained with TUNEL in Mut/Mut*** mice at 8 months of age with NMN or control (Scale bar, 20 μm). The number of TUNEL-positive cells per crypt is quantified on right (Data are presented as the mean ± S.D and n = 3 mice per group; unpaired two-tailed Student’s t-test). c Representative images of crypts at Day 0 and organoids at Day 8 from the intestine of Mut/Mut*** mice at 8 months of age with 200 μM NMN or water control (Scale bar, 100 μm). Organoid size and relative organoid number per well are quantified at bottom. Average organoid number per well is normalized to the control. Data are presented as the mean ± S.D of 6 wells from 3 mice per group; paired two-tailed Student’s t-test. Source data are provided with this paper.