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. 2024 Jan 16;15:546. doi: 10.1038/s41467-024-44808-z

Fig. 4. NAD+ depletion impairs Wnt/β-catenin signaling during the intestinal aging caused by mtDNA mutation burden.

Fig. 4

a Heatmap of differential genes associated with Wnt/β-catenin signaling in WT/WT*, WT/Mut** and Mut/Mut*** mice at 8 months of age (n = 3 mice per group). b Schematic illustration of LGR5-expressing ISCs, proliferated epithelial cells and Wnt/β-catenin signaling in the intestinal crypts. The figure of intestinal crypt was generated with the help of Servier Medical Art, provided by Servier, licensed under a Creative Commons Attribution 4.0 unported license (https://creativecommons.org/licenses/by/4.0/deed.en). c, d Anti-β-catenin (c) and anti-CD1 (d) IF in the intestinal crypts of WT/WT*, WT/Mut** and Mut/Mut*** mice at 3, 8 and 12 months of age. Relative FIs of β-catenin and CD1+ cell number per crypt are quantified on right (Scale bar, 10 μm; data are presented as the mean ± S.D and n = 3 mice per group; two-way ANOVA test). e Anti-β-catenin and anti-CD1 IF in the intestinal crypts of Mut/Mut*** mice at 8 months of age treated with NMN or water. Relative FI of β-catenin and CD1+ cell number per crypt are quantified on right (Scale bar, 10 μm; data are presented as the mean ± S.D and n = 3 mice per group; unpaired two-tailed Student’s t-test). Source data are provided with this paper.