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CT produced from pIG in classical strains
| Strain [relevant genotype] | CT productiona at pH:
|
|
|---|---|---|
| 6.5 | 8.5 | |
| O395 [wild type] | 7.1 | 0.03 |
| O395 (pIG) | 7.8 | 0.8 |
| JJM43 [O395 ΔtoxR] | <0.01 | <0.01 |
| JJM43 (pIG) | 0.5 | 0.8 |
| VJ740 [O395 ΔtoxT] | <0.01 | <0.01 |
| VJ740 (pIG) | 0.3 | 1.5 |
Cells were incubated in LB medium at 30°C at a starting pH of either 6.5 or 8.5. Toxin amounts are expressed in micrograms per unit of optical density at 600 nm. Values represent the averages of at least three independent experiments.
