Table 3.

CSNK1A1sgRNA design

Name	Sequence (5’–3’)
SM148.CRBN.g3 spacer	UGUAUGUGAUGUCGGCAGAC
CAGE1215.CSNK1A1.g1 spacer	UUCUAGUCGCCGAGAUGACA
CAGE1215.CSNK1A1.g7 spacer	UUUACCUUUAGCCCUUGCCA
Forward indexing primer (5’–3’)	AATGATACGGCGACCACCGAGATCTACAC(6 bp index)ACACTCTTTCCCTACACGACGCTCTTC
Reverse indexing primer (5’–3’)	CAAGCAGAAGACGGCATACGAGAT(10 bp index)GTGACTGGAGTTCAGACGTGTGCTC
SM148.hCRBN.DS.F2	ctacacgacgctcttccgatctGCAGAGAGTGAGGAAGAAGATGA
SM148.hCRBN.DS.R2	cagacgtgtgctcttccgatctGCCCATGTCCTCATCCACAA
CAGE1215.CSNK1A1.F	ctacacgacgctcttccgatctCACCAAATAGTGTTCCCTCCTCA
CAGE1215.CSNK1A1.R	cagacgtgtgctcttccgatctTGGGTACCAGCTTACTGTCTCT