FIG. 2.

ACP activation by liposomes containing LPS or PE. PC control liposomes were prepared with 2:1 molar ratios of DPPC and CHOL. LPS liposomes were prepared by addition of 12.5 μg of LPS of the indicated serotype/μmol of phospholipid. PE liposomes were prepared with 6:4:5 molar ratios of DPPC, DPPE, and CHOL. Liposomes (200 nmol of phospholipid) were incubated with MgEGTA-treated NHS, and C3 binding was measured by ELISA. Data presented are the mean ± SEM from three or more experiments.