TABLE 4.
Information on the identification of differential endogenous metabolites in the plasma of rats with blood deficiency (trend: model vs. control group).
| No. | TR (time) | Observed | VIP | Molecular formula | Metabolites | MS/MS fragment ion (m/z) | Trend |
|---|---|---|---|---|---|---|---|
| m/z (ESI+) | |||||||
| 1 | 11.28 | 560.3367 [M + FA + Li] + | 10.66 | C25H52NO7P | LysoPC(17:0) (Wang et al., 2022) | 86.0963, 104.1069, 184.0732, 500.2446, 524.3130, 542.3235 | ↑ |
| 2 | 10.82 | 818.5822 [M + Na]+ | 6.33 | C45H83N2O7P | SM(d20:1/20:4 (5Z,7E,11Z,14Z)-OH(9)) | 337.2517, 355.2627, 373.2733, 799.5704 | ↑ |
| 3 | 20.45 | 759.5700 [M + H]+ | 5.41 | C42H80NO8P | PC(18:1 (9Z)-O (12,13)/P-16:0) | 184.0732 | ↑ |
| 4 | 15.37 | 546.3528 [M + Na]+ | 4.45 | C26H54NO7P | LysoPC(0:0/18:0) | 86.0946, 91.0535, 184.0655, 238.0989, 261.1334 | ↑ |
| 5 | 9.54 | 305.2983 [M + H]+ | 4.4 | C20H32O2 | Arachidonic acid (Bian et al., 2023) | 44.9971 | ↑ |
| 6 | 15.48 | 611.4128 [M + Na]+ | 4.18 | C39H56O4 | Caffeoylcycloartenol | 89.0608, 207.0626, 269.2481 | ↓ |
| 7 | 15.31 | 613.4323 [M + CH3OH + H]+ | 3.47 | C33H56O8 | DG (PGE2/0:0/10:0) | 46.0655, 287.2359, 331.2636 | ↓ |
| 8 | 10.45 | 464.2823 [M + H]+ | 3.29 | C22H23O11 | LysoPA (20:2 (11Z,14Z)/0:0) | 97.9759,105.0332, 207.0816, 311.1207, 405.2041 | ↑ |
| 9 | 11.23 | 393.5709 [M + H]+ | 3.06 | C24H40O4 | Chenodeoxycholic acid (Zhan et al., 2012) | 82.0012, 128.1031 | ↓ |
| 10 | 8.98 | 162.1985 [M + H]+ | 2.92 | C7H15NO3 | L-Carnitine (Wu et al., 2016) | 74.0486, 89.0711 | ↓ |
| 11 | 14.78 | 759.5778 [M + H]+ | 2.04 | C42H80NO8P | PE (15:0/22:2 (13Z,16Z)) | 86.0964, 184.0730 | ↑ |
| 12 | 7.16 | 301.1412 [M + Na]+ | 1.78 | C16H22O4 | Diisobutyl phthalate | 45.0338, 67.0544, 89.0602 | ↑ |
| 13 | 1.06 | 90.0555 [M + H]+ | 1.73 | C3H7NO2 | L-Alanine (Huang et al., 2018) | 116.0936 | ↑ |
| 14 | 11.03 | 637.5191 [M + H]+ | 1.73 | C41H81NO3 | Ceramide (Li et al., 2022) | 74.0459, 312.2797, 398.3083 | ↑ |
| 15 | 6.32 | 176.0951 [M + H]+ | 1.66 | C6H13N3O3 | Citrulline (Zhang, 2020) | 143.7347, 156.9812 | ↓ |
| 16 | 8.53 | 129.0658 [M + H]+ | 1.65 | C5H8N2O2 | Dihydrothymine | 76.1312 | ↓ |
| 17 | 1.74 | 132.1735 [M + H]+ | 1.64 | C6H13NO2 | L-Leucine (Zhu et al., 2020) | 102.1044 | ↓ |
| 18 | 3.61 | 147.0773 [M + H]+ | 1.63 | C5H10N2O3 | L-Glutamine (Yang et al., 2013; Wang et al., 2015) | 155.4121 | ↓ |
| 19 | 15.59 | 482.2997 [M + H]+ | 1.52 | C28H37N2O5 | Unknown | 325.2018, 461.1941 | ↑ |