TABLE 2.
Summary of RT-qPCR results of 3-dpf angpt1–/– embryos.
| Biological process | Gene | angpt1 +/+ | angpt1 ± | angpt1 –/– | angpt1–/– vs. angpt1+/+ | ||||
| Mean | SD | Mean | SD | Mean | SD | p-value | |||
| Angiogenesis | angpt1 | 3.26E-03 | 3.33E-04 | 1.82E-03 | 1.13E-04 | 4.94E-04 | 6.77E-05 | P = 0.0036 | down* |
| angpt2a | 1.97E-04 | 3.05E-05 | 1.96E-04 | 1.43E-05 | 3.20E-04 | 6.66E-05 | P = 0.0714 | ns | |
| angpt2b | 4.74E-04 | 3.42E-05 | 5.07E-04 | 1.22E-05 | 5.24E-04 | 3.86E-05 | P = 0.3393 | ns | |
| figf | 1.03E-03 | 3.00E-05 | 1.12E-03 | 6.24E-05 | 1.40E-03 | 1.21E-04 | P = 0.0036 | up* | |
| itga5 | 5.61E-03 | 6.74E-04 | 5.11E-03 | 3.09E-04 | 4.62E-03 | 8.27E-04 | P = 0.2964 | ns | |
| itgav | 7.35E-02 | 2.91E-03 | 7.44E-02 | 4.46E-03 | 7.97E-02 | 2.23E-03 | P = 0.1321 | ns | |
| itgb1a | 3.80E-02 | 1.73E-03 | 3.71E-02 | 2.33E-03 | 4.15E-02 | 3.91E-03 | P = 0.2964 | ns | |
| itgb1b | 4.90E-02 | 4.16E-03 | 5.16E-02 | 2.61E-03 | 6.47E-02 | 4.63E-03 | P = 0.0500 | up* | |
| tek | 2.78E-04 | 5.50E-06 | 2.50E-04 | 1.22E-05 | 1.48E-04 | 2.00E-05 | P = 0.0036 | down* | |
| tie1 | 2.25E-03 | 2.66E-04 | 2.24E-03 | 2.11E-04 | 1.96E-03 | 1.43E-04 | P = 0.2321 | ns | |
| vegfaa | 6.57E-03 | 2.40E-04 | 6.22E-03 | 6.99E-05 | 8.21E-03 | 5.23E-04 | P = 0.0036 | up* | |
| vegfab | 4.84E-03 | 5.66E-04 | 4.18E-03 | 2.04E-04 | 4.40E-03 | 2.57E-04 | P = 0.1679 | ns | |
| vegfba | 2.53E-03 | 2.42E-04 | 2.23E-03 | 1.23E-04 | 1.65E-03 | 5.53E-05 | P = 0.0107 | down* | |
| vegfc | 1.07E-03 | 7.12E-05 | 1.00E-03 | 1.08E-04 | 1.12E-03 | 6.56E-05 | P = 0.2536 | ns | |
| Neural patterning | epha4a | 1.17E-02 | 7.58E-04 | 1.13E-02 | 3.99E-04 | 1.09E-02 | 3.67E-04 | P = 0.0857 | ns |
| ephb4a | 9.28E-03 | 4.81E-04 | 8.26E-03 | 4.43E-04 | 8.66E-03 | 4.27E-04 | P = 0.1321 | ns | |
| ephb4b | 5.10E-03 | 2.49E-04 | 4.77E-03 | 1.10E-04 | 5.02E-03 | 1.45E-04 | P = 0.1365 | ns | |
| sox2 | 3.14E-02 | 5.56E-04 | 2.98E-02 | 2.35E-03 | 2.05E-02 | 7.33E-04 | P = 0.0500 | down* | |
| sox10 | 3.43E-03 | 3.25E-04 | 2.98E-03 | 3.98E-04 | 2.70E-03 | 8.43E-04 | P = 0.4393 | ns | |
| wnt1 | 1.32E-03 | 2.10E-05 | 1.25E-03 | 1.27E-04 | 5.65E-04 | 1.11E-04 | P = 0.0250 | down* | |
| wnt2bb | 2.22E-03 | 5.24E-05 | 2.10E-03 | 2.32E-04 | 2.74E-03 | 2.57E-04 | P = 0.0500 | up* | |
| wnt10b | 8.33E-04 | 2.77E-05 | 8.38E-04 | 4.30E-05 | 4.81E-04 | 3.88E-05 | P = 0.0714 | ns | |
| Neuronal and glial development | apoea | 1.94E-01 | 2.61E-02 | 1.79E-01 | 1.92E-02 | 2.03E-01 | 9.53E-03 | P = 0.0714 | ns |
| apoeb | 2.71E-01 | 2.19E-02 | 2.47E-01 | 2.13E-02 | 3.32E-01 | 2.86E-02 | P = 0.0250 | up* | |
| gfap | 3.25E-02 | 3.75E-03 | 2.97E-02 | 3.59E-03 | 3.90E-02 | 1.62E-03 | P = 0.0500 | ns | |
| nes | 2.34E-03 | 2.47E-04 | 2.19E-03 | 2.58E-04 | 1.37E-03 | 2.13E-04 | P = 0.0250 | ns | |
| notch1a | 9.38E-03 | 1.78E-03 | 7.71E-03 | 5.66E-04 | 4.99E-03 | 4.23E-04 | P = 0.0107 | down* | |
| pax2a | 9.41E-03 | 9.27E-04 | 8.69E-03 | 1.19E-03 | 1.06E-02 | 1.37E-03 | P = 0.3821 | ns | |
| pax2b | 4.13E-03 | 5.03E-04 | 3.68E-03 | 2.72E-04 | 4.62E-03 | 4.06E-04 | P = 0.0857 | ns | |
| pax5 | 3.99E-03 | 5.12E-04 | 3.67E-03 | 4.29E-04 | 1.96E-03 | 1.78E-04 | P = 0.0700 | ns | |
| pcna | 1.17E-01 | 2.20E-03 | 9.61E-02 | 1.58E-03 | 4.55E-02 | 1.38E-02 | P = 0.0219 | down* | |
| hdc | 1.16E-03 | 1.15E-04 | 1.05E-03 | 9.94E-05 | 1.02E-04 | 1.19E-04 | P = 0.3607 | ns | |
| Th | 2.83E-03 | 1.80E-04 | 2.72E-03 | 7.05E-05 | 2.17E-03 | 1.89E-04 | P = 0.0760 | ns | |
| th2 | 2.87E-05 | 6.76E-06 | 2.92E-05 | 3.56E-06 | 4.05E-05 | 1.43E-05 | P = 0.6991 | ns | |
Data are mean ± SD. Kruskal–Wallis test with Dunn’s multiple comparisons test was used for statistical analysis *p < 0.05, ns: non-significant, down: downregulation in the angpt1–/– compared with the angp1+/+ sibling, up: upregulation in the angpt1–/– compared with the angp1+/+ sibling.