Table 1.
Components of the External Quality Assessment (EQA) specimen panel.
| Specimen | Copies of SARS-CoV-2 per mLa or Cq value for not-quantified pathogens | Specimens used in EQA round | Strain, source |
|---|---|---|---|
| SARS-CoV-2 (SARS2_H) | 1.28 × 105 | 1, 2, 3 | hCoV-19/Netherlands/NoordBrabant_10003/2020, RIVM |
| SARS-CoV-2 (SARS2_L)b | 1.28 × 103 | 1, 2, 3 | |
| SARS-CoV-2 (SARS2_Edu) | 1.28 × 102 | 1, 2, 3 | |
| SARS-CoV-2 Alpha_H | 3.39 × 105 | 2 | hCoV-19/Netherlands/NH-RIVM-20432/2020 B.1.1.7 20B/501Y.V1 , RIVM |
| SARS-CoV-2 Alpha_L | 3.39 × 104 | 3 | |
| SARS-CoV-2 Beta | 1.15 × 104 | 3 | hCoV-19/Netherlands/NH-RIVM-10159/2021, RIVM |
| SARS-CoV-2 Gamma | 8.77 × 103 | 3 | |
| SARS-CoV-1 | Cq 28.57 (Sarbeco E-gene) | 1, 2, 3 | HKU-39849, kindly provided by Bart Haagmans, Erasmus MC |
| hCoV-NL63 | Cq 28.10 (N-gene) | 1, 2, 3 | Clinical isolate, kindly provided by Lia van der Hoek, Amsterdam University Medical Center |
| hCoV-229E | Cq 17.22 (N-gene) | 1, 2 | ATCC VR-740, ATCC, Manassas, Virginia |
| hCoV-OC43 | Cq 27.77 (N-gene) | 1, 2 | ATCC VR-1558, ATCC, Manassas, Virginia |
| Influenza A (H3N2) | Cq 22.76 (M-gene) | 1 | A/Netherlands/10,078/2020, RIVM |
| No virus control | n/a | 1,2,3 | n/a |